Determination of complex type free, non‐conjugated Oligosaccharide Glucose Unit values in tomato xylem sap for early detection of nutrient deficiency

Genesis-Sustainable-Future-Ltd.-Electrophoresis

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Abstract

Although knowledge on glycan biosynthesis and processing is continuously maturing, there are still a limited number of studies that examine the biological functions of N-glycan structures in plants, which remain virtually unknown.

Here, the statistical correlation between nutrient (nitrogen) deficiency symptoms of crops and changes in 8-aminopyrene-1,3,6-trisulfonic acid (APTS)-labeled complex type free oligosaccharides is reported. While deficiency symptoms are predicted by multispectral images and Kjeldahl digestion, APTS-labeled complex type free oligosaccharides are identified by their glucose unit (GU) values in tomato xylem sap, using capillary electrophoresis with laser-induced fluorescence detection (CE-LIF). Given the limited number of structures obtained from plants, archived in the literature, in the future, it is intended to create an open-access database of promising indicators, namely, glycan structures that are presumably responsible for the nutrient deficiency caused stress in plants.

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