GlycoPlants is a project with the purpose of building up an Open Access database to store and share glycan retention properties of structures that are conditionally associated with plant stress caused by nutrient deficiency or crop’s diseases

Problem we focus on

Currently there is a negligible amount of open access Glycan attributes such as profiles available in the literature, (e.g only 4 glycan profiles detected in plant samples are published in the GlycoStore database, which is widely accepted in the field). That’s unfortunate, since glycosylation is very important in plant science  considering that it can modify various biological functions of proteins, and affect growth, physiology, and development of crops. It seemed to be confirmed by our basic research in which we found statistical correlations between nutrient deficiency symptoms of crops  and changes in suspected PCT-FNGs profiles based only on GU values, observed by CE-LIF.

Papers

Mátyás, B., Singer, J., Szarka, M., Lowy, D.A., Döncző, B., Makleit, P., Failoc‐Rojas, V.E., Ramirez, A., Martínez, P., Sándor, Z., Kincses, I. and Guttman, A. (2020), Determination of complex type free, non‐conjugated Oligosaccharide Glucose Unit values in tomato xylem sap for early detection of nutrient deficiency. ELECTROPHORESIS. Accepted Author Manuscript. doi:10.1002/elps.202000254

Beta

◀️Responsive Database I Slide screen for more records ▶️

As of current GlycoPlants consists: Suspected structures being linked to reactions to stress caused by nutrient (N) deficiency in plants based on our separation in tomato xylem sap; and exact GU matches consulted with GlycoStore.org; and Complex type free N-glycan detected mostly by HPLC (extracted from literature, please find DOI(s) in Reference column; and we periodically extract plant data (if any) from Glycostore. Last Update: 18 May, 2020

ID	Structure	GU	Glycan	RT Type	Associated diseases/nutrient deficiency	sample	Sample age	Variotype	Origin	References	Equipment	Detector type	Buffer	Separation temperature (℃)	Lead researcher	Institution	Contact
1	A3G(4)3S(3,6,3,6)4	4.19		CE	N deficiency	Tomato	55 days old seedlings	Solanum lycopersicum	Ecuador	DRC preliminary	Proteomelab CE, with Laser induced fluorescence detector module	405 nm diode laser. Cartridge with 50 cm effective length (60 cm total length) bare fused silica (BFS) capillary with 50 µm inner (ID) diameter. Filtration: 520 nm bandpass (Beckman-Coulter, Fullerton, CA, USA)	HR-NCHO separation gel	30	Mátyás,B	Research Group of Applied Plant Glycobiology, Dama Research Center limited	bmatyas@edu.damaresearch.com
2	A3G(4)3S(6,6,3)3	4.92	n.a.	CE	N deficiency	Tomato	55 days old seedlings	Solanum lycopersicum	Ecuador	DRC preliminary	Proteomelab CE, with Laser induced fluorescence detector module	405 nm diode laser. Cartridge with 50 cm effective length (60 cm total length) bare fused silica (BFS) capillary with 50 µm inner (ID) diameter. Filtration: 520 nm bandpass (Beckman-Coulter, Fullerton, CA, USA)	HR-NCHO separation gel	30	Mátyás,B	Research Group of Applied Plant Glycobiology, Dama Research Center limited	bmatyas@edu.damaresearch.com
3	A2BG2	9.84		CE	N deficiency	Tomato	55 days old seedlings	Solanum lycopersicum	Ecuador	DRC preliminary	Proteomelab CE, with Laser induced fluorescence detector module	405 nm diode laser. Cartridge with 50 cm effective length (60 cm total length) bare fused silica (BFS) capillary with 50 µm inner (ID) diameter. Filtration: 520 nm bandpass (Beckman-Coulter, Fullerton, CA, USA)	HR-NCHO separation gel	30	Mátyás,B	Research Group of Applied Plant Glycobiology, Dama Research Center limited	bmatyas@edu.damaresearch.com
4	G1F1GN2M3FX	n.a.		HPLC	n.a.	Tomato	55 days old seedlings	KGM 172, Kagome Co. Tochigi, Japan	Japan	10.1080/09168451.2019.1608803	RP-HPLC; usingCosmosil 5C18 AR column (6.0 x 250 mm, Nacalai Tesque, Kyoto)	Filtration: Gel with Sephadex G-25 superfine column (1.5 x 16 cm) in 0.1 N NH4OH,	n.a.	n.a.	Yoshinobu Kimura	Graduate School of Environmental and Life Science, Okayama University	josh8mar@okayama-u.ac.jp
5	G1GN2M3FX	n.a.		HPLC	n.a.	Tomato	55 days old seedlings	KGM 172, Kagome Co. Tochigi, Japan	Japan	10.1080/09168451.2019.1608803	RP-HPLC; usingCosmosil 5C18 AR column (6.0 x 250 mm, Nacalai Tesque, Kyoto)	Filtration: Gel with Sephadex G-25 superfine column (1.5 x 16 cm) in 0.1 N NH4OH,	n.a.	n.a.	Yoshinobu Kimura	Graduate School of Environmental and Life Science, Okayama University	josh8mar@okayama-u.ac.jp
6	GN2M3FX	n.a.		HPLC	n.a.	Tomato	55 days old seedlings	KGM 172, Kagome Co. Tochigi, Japan	Japan	10.1080/09168451.2019.1608803	RP-HPLC; usingCosmosil 5C18 AR column (6.0 x 250 mm, Nacalai Tesque, Kyoto)	Gel with Sephadex G-25 superfine column (1.5 x 16 cm) in 0.1 N NH4OH,	n.a.	n.a.	Yoshinobu Kimura	Graduate School of Environmental and Life Science, Okayama University	josh8mar@okayama-u.ac.jp
7	M3FX	n.a.		HPLC	n.a.	Tomato	55 days old seedlings	KGM 172, Kagome Co. Tochigi, Japan	Japan	10.1080/09168451.2019.1608803	RP-HPLC; usingCosmosil 5C18 AR column (6.0 x 250 mm, Nacalai Tesque, Kyoto)	Gel with Sephadex G-25 superfine column (1.5 x 16 cm) in 0.1 N NH4OH,	n.a.	n.a.	Yoshinobu Kimura	Graduate School of Environmental and Life Science, Okayama University	josh8mar@okayama-u.ac.jp